pgem1-t easy vector (Promega)
90
Structured Review
Promega
pgem1-t easy vector
Pgem1 T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem1-t+easy+vector/pgem+t+easy/pm36490276-109-15-18
Average 90 stars, based on 1 article reviews
Pgem1 T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem1-t+easy+vector/pgem+t+easy/pm36490276-109-15-18
Average 90 stars, based on 1 article reviews
pgem1-t easy vector - by Bioz Stars,
2026-11
90/100 stars
Images
Related Articles
Polymerase Chain Reaction:Article Title: The colorful mantle of the giant clam Tridacna squamosa expresses a homolog of electrogenic sodium: Bicarbonate cotransporter 2 that mediates the supply of inorganic carbon to photosynthesizing symbionts. Article Snippet: Then, the band-of-interest was excised and purified using Article Title: Seroepidemiology and occult hepatitis B virus infection in young adults in Banjarmasin, Indonesia. Article Snippet: Hepatitis B virus (HBV) infection remains a public health problem in Indonesia.. There has been limited data regarding HBV infection in young adult population.. This study aimed to evaluate the seroepidemiology of HBV infection and characterize occult HBV variants in healthy young adults in Banjarmasin, Indonesia, who were born before the implementation of the universal infant hepatitis B vaccination. Article Title: Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates. Article Snippet: Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient.. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE).. However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. Article Title: Detection and quantification of bovine papillomavirus type 2 in urinary bladders and lymph nodes in cases of Bovine Enzootic Hematuria from the endemic region of Azores. Article Snippet: Bovine Enzootic Hematuria (BEH) is a disease with a severe impact on production indexes and characterized by the development of bovine urinary bladder tumors, particularly in the Azores archipelago.. The purpose of this study was to investigate and quantify BPV2 tissue distribution in bovine urinary bladder tumors, normal bladders, and iliac lymph nodes of cattle from the Azores.. A real-time PCR system targeting the L1 gene was developed and allowed for the specific detection of the virus. Article Title: Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer. Article Snippet: After amplification, each product was ligated to pGEM1-T Easy Vector (Promega, WI, USA) according to the PLOS ONE | https://doi.org/10.1371/journal.pone.0233784 June 3, 2020 3 / 18 manufacturer’s instructions and verified by sequencing analysis (BIONICS Corp., Seoul, Korea). Article Title: Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. Article Snippet: The PCR product amplified from LF5HA92 could not be sequenced directly and was cloned into pGEM1-T easy vector (Promega, Catalog number A1360) and sequenced. Article Title: Screening of cell wall-related genes that are expressed differentially during ripening of pears with different softening characteristics Article Snippet: Postharvest softening is an important physiological process that influences pear fruit quality.. ‘Starkrimson’ pears soften rapidly, resulting in fruits with a low tolerance for storage and transportation.. To better characterize the mechanism regulating the rapid softening of pears, cell wall-related changes were compared among three soft-fleshed and rapid-softening cultivars (‘Starkrimson’, ‘Bartlett’, and ‘Abbe Fetel’) and a crisp-fleshed and non-softening cultivar (‘Dangshansuli’). Amplification:Article Title: The colorful mantle of the giant clam Tridacna squamosa expresses a homolog of electrogenic sodium: Bicarbonate cotransporter 2 that mediates the supply of inorganic carbon to photosynthesizing symbionts. Article Snippet: Then, the band-of-interest was excised and purified using Article Title: Seroepidemiology and occult hepatitis B virus infection in young adults in Banjarmasin, Indonesia. Article Snippet: Hepatitis B virus (HBV) infection remains a public health problem in Indonesia.. There has been limited data regarding HBV infection in young adult population.. This study aimed to evaluate the seroepidemiology of HBV infection and characterize occult HBV variants in healthy young adults in Banjarmasin, Indonesia, who were born before the implementation of the universal infant hepatitis B vaccination. Article Title: Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates. Article Snippet: Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient.. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE).. However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. Article Title: Detection and quantification of bovine papillomavirus type 2 in urinary bladders and lymph nodes in cases of Bovine Enzootic Hematuria from the endemic region of Azores. Article Snippet: Bovine Enzootic Hematuria (BEH) is a disease with a severe impact on production indexes and characterized by the development of bovine urinary bladder tumors, particularly in the Azores archipelago.. The purpose of this study was to investigate and quantify BPV2 tissue distribution in bovine urinary bladder tumors, normal bladders, and iliac lymph nodes of cattle from the Azores.. A real-time PCR system targeting the L1 gene was developed and allowed for the specific detection of the virus. Article Title: Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer. Article Snippet: After amplification, each product was ligated to pGEM1-T Easy Vector (Promega, WI, USA) according to the PLOS ONE | https://doi.org/10.1371/journal.pone.0233784 June 3, 2020 3 / 18 manufacturer’s instructions and verified by sequencing analysis (BIONICS Corp., Seoul, Korea). Article Title: Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. Article Snippet: The PCR product amplified from LF5HA92 could not be sequenced directly and was cloned into pGEM1-T easy vector (Promega, Catalog number A1360) and sequenced. Article Title: Screening of cell wall-related genes that are expressed differentially during ripening of pears with different softening characteristics Article Snippet: Postharvest softening is an important physiological process that influences pear fruit quality.. ‘Starkrimson’ pears soften rapidly, resulting in fruits with a low tolerance for storage and transportation.. To better characterize the mechanism regulating the rapid softening of pears, cell wall-related changes were compared among three soft-fleshed and rapid-softening cultivars (‘Starkrimson’, ‘Bartlett’, and ‘Abbe Fetel’) and a crisp-fleshed and non-softening cultivar (‘Dangshansuli’). Clone Assay:Article Title: The colorful mantle of the giant clam Tridacna squamosa expresses a homolog of electrogenic sodium: Bicarbonate cotransporter 2 that mediates the supply of inorganic carbon to photosynthesizing symbionts. Article Snippet: Then, the band-of-interest was excised and purified using Article Title: Seroepidemiology and occult hepatitis B virus infection in young adults in Banjarmasin, Indonesia. Article Snippet: Hepatitis B virus (HBV) infection remains a public health problem in Indonesia.. There has been limited data regarding HBV infection in young adult population.. This study aimed to evaluate the seroepidemiology of HBV infection and characterize occult HBV variants in healthy young adults in Banjarmasin, Indonesia, who were born before the implementation of the universal infant hepatitis B vaccination. Article Title: Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates. Article Snippet: Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient.. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE).. However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. Article Title: Detection and quantification of bovine papillomavirus type 2 in urinary bladders and lymph nodes in cases of Bovine Enzootic Hematuria from the endemic region of Azores. Article Snippet: Bovine Enzootic Hematuria (BEH) is a disease with a severe impact on production indexes and characterized by the development of bovine urinary bladder tumors, particularly in the Azores archipelago.. The purpose of this study was to investigate and quantify BPV2 tissue distribution in bovine urinary bladder tumors, normal bladders, and iliac lymph nodes of cattle from the Azores.. A real-time PCR system targeting the L1 gene was developed and allowed for the specific detection of the virus. Article Title: Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer. Article Snippet: After amplification, each product was ligated to pGEM1-T Easy Vector (Promega, WI, USA) according to the PLOS ONE | https://doi.org/10.1371/journal.pone.0233784 June 3, 2020 3 / 18 manufacturer’s instructions and verified by sequencing analysis (BIONICS Corp., Seoul, Korea). Article Title: Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. Article Snippet: The PCR product amplified from LF5HA92 could not be sequenced directly and was cloned into pGEM1-T easy vector (Promega, Catalog number A1360) and sequenced. Article Title: Screening of cell wall-related genes that are expressed differentially during ripening of pears with different softening characteristics Article Snippet: Postharvest softening is an important physiological process that influences pear fruit quality.. ‘Starkrimson’ pears soften rapidly, resulting in fruits with a low tolerance for storage and transportation.. To better characterize the mechanism regulating the rapid softening of pears, cell wall-related changes were compared among three soft-fleshed and rapid-softening cultivars (‘Starkrimson’, ‘Bartlett’, and ‘Abbe Fetel’) and a crisp-fleshed and non-softening cultivar (‘Dangshansuli’). Plasmid Preparation:Article Title: The colorful mantle of the giant clam Tridacna squamosa expresses a homolog of electrogenic sodium: Bicarbonate cotransporter 2 that mediates the supply of inorganic carbon to photosynthesizing symbionts. Article Snippet: Then, the band-of-interest was excised and purified using Article Title: Seroepidemiology and occult hepatitis B virus infection in young adults in Banjarmasin, Indonesia. Article Snippet: Hepatitis B virus (HBV) infection remains a public health problem in Indonesia.. There has been limited data regarding HBV infection in young adult population.. This study aimed to evaluate the seroepidemiology of HBV infection and characterize occult HBV variants in healthy young adults in Banjarmasin, Indonesia, who were born before the implementation of the universal infant hepatitis B vaccination. Article Title: Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates. Article Snippet: Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient.. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE).. However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. Article Title: Detection and quantification of bovine papillomavirus type 2 in urinary bladders and lymph nodes in cases of Bovine Enzootic Hematuria from the endemic region of Azores. Article Snippet: Bovine Enzootic Hematuria (BEH) is a disease with a severe impact on production indexes and characterized by the development of bovine urinary bladder tumors, particularly in the Azores archipelago.. The purpose of this study was to investigate and quantify BPV2 tissue distribution in bovine urinary bladder tumors, normal bladders, and iliac lymph nodes of cattle from the Azores.. A real-time PCR system targeting the L1 gene was developed and allowed for the specific detection of the virus. Article Title: Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer. Article Snippet: After amplification, each product was ligated to pGEM1-T Easy Vector (Promega, WI, USA) according to the PLOS ONE | https://doi.org/10.1371/journal.pone.0233784 June 3, 2020 3 / 18 manufacturer’s instructions and verified by sequencing analysis (BIONICS Corp., Seoul, Korea). Article Title: Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. Article Snippet: The PCR product amplified from LF5HA92 could not be sequenced directly and was cloned into pGEM1-T easy vector (Promega, Catalog number A1360) and sequenced. Article Title: Screening of cell wall-related genes that are expressed differentially during ripening of pears with different softening characteristics Article Snippet: Postharvest softening is an important physiological process that influences pear fruit quality.. ‘Starkrimson’ pears soften rapidly, resulting in fruits with a low tolerance for storage and transportation.. To better characterize the mechanism regulating the rapid softening of pears, cell wall-related changes were compared among three soft-fleshed and rapid-softening cultivars (‘Starkrimson’, ‘Bartlett’, and ‘Abbe Fetel’) and a crisp-fleshed and non-softening cultivar (‘Dangshansuli’). Sequencing:Article Title: The colorful mantle of the giant clam Tridacna squamosa expresses a homolog of electrogenic sodium: Bicarbonate cotransporter 2 that mediates the supply of inorganic carbon to photosynthesizing symbionts. Article Snippet: Then, the band-of-interest was excised and purified using Article Title: Seroepidemiology and occult hepatitis B virus infection in young adults in Banjarmasin, Indonesia. Article Snippet: Hepatitis B virus (HBV) infection remains a public health problem in Indonesia.. There has been limited data regarding HBV infection in young adult population.. This study aimed to evaluate the seroepidemiology of HBV infection and characterize occult HBV variants in healthy young adults in Banjarmasin, Indonesia, who were born before the implementation of the universal infant hepatitis B vaccination. Article Title: Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates. Article Snippet: Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient.. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE).. However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. Article Title: Detection and quantification of bovine papillomavirus type 2 in urinary bladders and lymph nodes in cases of Bovine Enzootic Hematuria from the endemic region of Azores. Article Snippet: Bovine Enzootic Hematuria (BEH) is a disease with a severe impact on production indexes and characterized by the development of bovine urinary bladder tumors, particularly in the Azores archipelago.. The purpose of this study was to investigate and quantify BPV2 tissue distribution in bovine urinary bladder tumors, normal bladders, and iliac lymph nodes of cattle from the Azores.. A real-time PCR system targeting the L1 gene was developed and allowed for the specific detection of the virus. Article Title: Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer. Article Snippet: After amplification, each product was ligated to pGEM1-T Easy Vector (Promega, WI, USA) according to the PLOS ONE | https://doi.org/10.1371/journal.pone.0233784 June 3, 2020 3 / 18 manufacturer’s instructions and verified by sequencing analysis (BIONICS Corp., Seoul, Korea). Article Title: Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. Article Snippet: The PCR product amplified from LF5HA92 could not be sequenced directly and was cloned into pGEM1-T easy vector (Promega, Catalog number A1360) and sequenced. Article Title: Screening of cell wall-related genes that are expressed differentially during ripening of pears with different softening characteristics Article Snippet: Postharvest softening is an important physiological process that influences pear fruit quality.. ‘Starkrimson’ pears soften rapidly, resulting in fruits with a low tolerance for storage and transportation.. To better characterize the mechanism regulating the rapid softening of pears, cell wall-related changes were compared among three soft-fleshed and rapid-softening cultivars (‘Starkrimson’, ‘Bartlett’, and ‘Abbe Fetel’) and a crisp-fleshed and non-softening cultivar (‘Dangshansuli’). Infection:Article Title: The colorful mantle of the giant clam Tridacna squamosa expresses a homolog of electrogenic sodium: Bicarbonate cotransporter 2 that mediates the supply of inorganic carbon to photosynthesizing symbionts. Article Snippet: Then, the band-of-interest was excised and purified using Article Title: Seroepidemiology and occult hepatitis B virus infection in young adults in Banjarmasin, Indonesia. Article Snippet: Hepatitis B virus (HBV) infection remains a public health problem in Indonesia.. There has been limited data regarding HBV infection in young adult population.. This study aimed to evaluate the seroepidemiology of HBV infection and characterize occult HBV variants in healthy young adults in Banjarmasin, Indonesia, who were born before the implementation of the universal infant hepatitis B vaccination. Article Title: Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates. Article Snippet: Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient.. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE).. However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. Article Title: Detection and quantification of bovine papillomavirus type 2 in urinary bladders and lymph nodes in cases of Bovine Enzootic Hematuria from the endemic region of Azores. Article Snippet: Bovine Enzootic Hematuria (BEH) is a disease with a severe impact on production indexes and characterized by the development of bovine urinary bladder tumors, particularly in the Azores archipelago.. The purpose of this study was to investigate and quantify BPV2 tissue distribution in bovine urinary bladder tumors, normal bladders, and iliac lymph nodes of cattle from the Azores.. A real-time PCR system targeting the L1 gene was developed and allowed for the specific detection of the virus. Article Title: Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer. Article Snippet: After amplification, each product was ligated to pGEM1-T Easy Vector (Promega, WI, USA) according to the PLOS ONE | https://doi.org/10.1371/journal.pone.0233784 June 3, 2020 3 / 18 manufacturer’s instructions and verified by sequencing analysis (BIONICS Corp., Seoul, Korea). Article Title: Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. Article Snippet: The PCR product amplified from LF5HA92 could not be sequenced directly and was cloned into pGEM1-T easy vector (Promega, Catalog number A1360) and sequenced. Article Title: Screening of cell wall-related genes that are expressed differentially during ripening of pears with different softening characteristics Article Snippet: Postharvest softening is an important physiological process that influences pear fruit quality.. ‘Starkrimson’ pears soften rapidly, resulting in fruits with a low tolerance for storage and transportation.. To better characterize the mechanism regulating the rapid softening of pears, cell wall-related changes were compared among three soft-fleshed and rapid-softening cultivars (‘Starkrimson’, ‘Bartlett’, and ‘Abbe Fetel’) and a crisp-fleshed and non-softening cultivar (‘Dangshansuli’). Ligation:Article Title: The colorful mantle of the giant clam Tridacna squamosa expresses a homolog of electrogenic sodium: Bicarbonate cotransporter 2 that mediates the supply of inorganic carbon to photosynthesizing symbionts. Article Snippet: Then, the band-of-interest was excised and purified using Article Title: Seroepidemiology and occult hepatitis B virus infection in young adults in Banjarmasin, Indonesia. Article Snippet: Hepatitis B virus (HBV) infection remains a public health problem in Indonesia.. There has been limited data regarding HBV infection in young adult population.. This study aimed to evaluate the seroepidemiology of HBV infection and characterize occult HBV variants in healthy young adults in Banjarmasin, Indonesia, who were born before the implementation of the universal infant hepatitis B vaccination. Article Title: Intrapersonal mutation of rmpA and rmpA2: A reason for negative hypermucoviscosity phenotype and low virulence of rmpA-positive Klebsiella pneumoniae isolates. Article Snippet: Two Klebsiella pneumoniae isolates were simultaneously recovered from blood and urine cultures of the same patient.. Both isolates were identical in genomic pulsotype by pulsed-field gel electrophoresis (PFGE).. However, the hypermucoviscosity phenotype was confirmed in the blood strain but not the urine strain. Article Title: Detection and quantification of bovine papillomavirus type 2 in urinary bladders and lymph nodes in cases of Bovine Enzootic Hematuria from the endemic region of Azores. Article Snippet: Bovine Enzootic Hematuria (BEH) is a disease with a severe impact on production indexes and characterized by the development of bovine urinary bladder tumors, particularly in the Azores archipelago.. The purpose of this study was to investigate and quantify BPV2 tissue distribution in bovine urinary bladder tumors, normal bladders, and iliac lymph nodes of cattle from the Azores.. A real-time PCR system targeting the L1 gene was developed and allowed for the specific detection of the virus. Article Title: Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer. Article Snippet: After amplification, each product was ligated to pGEM1-T Easy Vector (Promega, WI, USA) according to the PLOS ONE | https://doi.org/10.1371/journal.pone.0233784 June 3, 2020 3 / 18 manufacturer’s instructions and verified by sequencing analysis (BIONICS Corp., Seoul, Korea). Article Title: Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. Article Snippet: The PCR product amplified from LF5HA92 could not be sequenced directly and was cloned into pGEM1-T easy vector (Promega, Catalog number A1360) and sequenced. Article Title: Screening of cell wall-related genes that are expressed differentially during ripening of pears with different softening characteristics Article Snippet: Postharvest softening is an important physiological process that influences pear fruit quality.. ‘Starkrimson’ pears soften rapidly, resulting in fruits with a low tolerance for storage and transportation.. To better characterize the mechanism regulating the rapid softening of pears, cell wall-related changes were compared among three soft-fleshed and rapid-softening cultivars (‘Starkrimson’, ‘Bartlett’, and ‘Abbe Fetel’) and a crisp-fleshed and non-softening cultivar (‘Dangshansuli’). |